Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2009-09-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0809 |
Spacegroup name | P 63 |
Unit cell lengths | 83.967, 83.967, 115.724 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 1.990 |
R-factor | 0.1635 |
Rwork | 0.162 |
R-free | 0.19020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eoj |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.050 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.186 | 0.580 |
Number of reflections | 29980 | |
<I/σ(I)> | 1.2 | |
Completeness [%] | 94.5 | 94 |
Redundancy | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 mM HEPES, pH 7.5, 16% PEG2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 293K |