3VD7
E. coli (lacZ) beta-galactosidase (N460S) in complex with galactotetrazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 128.460, 152.370, 135.110 |
| Unit cell angles | 90.00, 104.25, 90.00 |
Refinement procedure
| Resolution | 44.780 - 2.870 |
| R-factor | 0.19044 |
| Rwork | 0.188 |
| R-free | 0.25016 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3vd5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.135 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.080 | 0.269 |
| Number of reflections | 107407 | |
| <I/σ(I)> | 10.95 | 2.44 |
| Completeness [%] | 93.7 | 98 |
| Redundancy | 2.06 | 1.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 10% PEG8000, 100 mM Bis-Tris, pH 6.5, 200 mM magnesium chloride, 100 mM sodium chloride, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
