3VC5
Crystal structure of enolase Tbis_1083(TARGET EFI-502310) FROM Thermobispora bispora DSM 43833 complexed with phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 78.092, 78.092, 165.691 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| R-factor | 0.14335 |
| Rwork | 0.142 |
| R-free | 0.17675 |
| Structure solution method | MR |
| Starting model (for MR) | 3mzn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.352 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.940 | |
| Number of reflections | 82238 | |
| <I/σ(I)> | 7.4 | 1.4 |
| Completeness [%] | 98.6 | 98.8 |
| Redundancy | 11 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 0.2M LITHIUM SULFATE, PH 8.5, 0.1M TRIS-HCL, 30% PEG4000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
