3VC0
Crystal structure of Taipoxin beta subunit isoform 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.91232 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.890, 76.860, 104.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.970 - 2.150 |
| R-factor | 0.19156 |
| Rwork | 0.189 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ae7 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.791 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.970 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.117 | 0.443 |
| Number of reflections | 8636 | |
| <I/σ(I)> | 6.3 | 1.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.05 M Cadmium sulfate, 0.1 M HEPES Sodium salt, 1.0 M Sodium acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
