3VB8
Crystal Structure of Engineered Protein, Northeast Structural Genomics Consortium Target OR43
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-10 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.553, 77.553, 115.741 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.680 - 2.900 |
R-factor | 0.244 |
Rwork | 0.244 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ii3 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.135 | |
Number of reflections | 9162 | |
<I/σ(I)> | 10.4 | |
Completeness [%] | 93.5 | 98.3 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 293 | 1.59M MgSO4, 0.1 M Tris, pH 8, Microbatch under oil, temperature 293K |