3VAZ

Crystal structure of Staphylococcal GAPDH1 in a hexagonal space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-09-25
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 62 2 2
Unit cell lengths	155.389, 155.389, 317.849
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.740 - 3.190
R-factor	0.2059
Rwork	0.202
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k73
RMSD bond length	0.006
RMSD bond angle	0.995
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.742		3.390
High resolution limit [Å]	3.190	9.170	3.190
Rmerge	0.315	0.070	0.733
Rmeas		0.072	0.751
Number of reflections	38297	1771	5952
<I/σ(I)>	13.8	40.57	5.35
Completeness [%]	99.6	95.7	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	22% PEG 400, 0.2M CaCl2, 0.1M HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 298K