3VAC
Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.066, 126.364, 78.701 |
| Unit cell angles | 90.00, 100.49, 90.00 |
Refinement procedure
| Resolution | 46.777 - 2.600 |
| R-factor | 0.1861 |
| Rwork | 0.184 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.712 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.098 |
| Number of reflections | 33308 |
| Completeness [%] | 95.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 288 | 0.1 M sodium citrate, pH 5.8, 1.5 M sodium chloride, 18% PEG4000, 50 mM guanidine chloride, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
