3VA9

Crystal structure of the Rhodopseudomonas palustris histidine kinase HK9 sensor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-16
Detector	ADSC QUANTUM 4r
Wavelength(s)	1.7432
Spacegroup name	I 41 2 2
Unit cell lengths	76.147, 76.147, 109.155
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.074 - 2.300
R-factor	0.1891
Rwork	0.187
R-free	0.22660
Structure solution method	Native SAD
RMSD bond length	0.005
RMSD bond angle	0.770
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXD
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.360
High resolution limit [Å]	2.300	2.300
Number of reflections	7462
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	273	18% PEG 1K, 100mM Cacodylate, pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K