3V9R
Crystal structure of Saccharomyces cerevisiae MHF complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-29 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 68.823, 104.065, 70.862 |
| Unit cell angles | 90.00, 109.09, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.2421 |
| Rwork | 0.239 |
| R-free | 0.29040 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.084 | 0.358 |
| Number of reflections | 18254 | |
| <I/σ(I)> | 47.6 | 6.8 |
| Completeness [%] | 98.6 | 98 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.6M (NH4)2SO4, 3% isopropanol, 8% polypropylene glycol P400, 0.02M CaCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
