3V9M
Phospholipase ACII4 from Australian King Brown Snake
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 |
| Unit cell lengths | 82.380, 82.380, 28.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.455 - 1.563 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.22540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.028 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.3_473)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.440 | 1.609 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 28491 | |
| Completeness [%] | 96.8 | 93.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | 0.2M LITHIUM SULFATE MONOHYDRATE, 0.1M TRIS-HCL, 30% (W/V) PEG 4000, , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
