3V9F
Crystal Structure of the extracellular domain of the putative hybrid two component system BT3049 from B. thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.927, 114.117, 487.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.830 - 3.300 |
| R-factor | 0.2277 |
| Rwork | 0.225 |
| R-free | 0.27670 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.610 |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 3.300 |
| Number of reflections | 68485 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 5% PEGMME 2K, 0.1 M Tris buffer pH7.0, vapor diffusion, temperature 293K |
| 1 | VAPOR DIFFUSION | 7 | 293 | 5% PEGMME 2K, 0.1 M Tris buffer pH7.0, vapor diffusion, temperature 293K |
