3V9A

Crystal structure of Esterase/Lipase from uncultured bacterium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-20
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	61.060, 61.060, 149.117
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.240 - 2.070
R-factor	0.1922
Rwork	0.187
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.916
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.070	2.070
Number of reflections	16720
Completeness [%]	92.7	84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	0.1M Tris, pH 7.0, 2.0M ammonium sulfate, 0.2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K