3V8V

Crystal structure of bifunctional methyltransferase YcbY (RlmLK) from Escherichia coli, SAM binding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-01
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 1 21 1
Unit cell lengths	73.724, 140.586, 102.097
Unit cell angles	90.00, 101.88, 90.00

Refinement procedure

Resolution	42.570 - 2.600
R-factor	0.18544
Rwork	0.182
R-free	0.24694
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b78 3ldg 3ldf
RMSD bond length	0.018
RMSD bond angle	1.961
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Number of reflections	58938
Completeness [%]	94.7	99.7	76.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	0.1M HEPES, 12%(w/v) PEG 8000 , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K