3V8V
Crystal structure of bifunctional methyltransferase YcbY (RlmLK) from Escherichia coli, SAM binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.724, 140.586, 102.097 |
| Unit cell angles | 90.00, 101.88, 90.00 |
Refinement procedure
| Resolution | 42.570 - 2.600 |
| R-factor | 0.18544 |
| Rwork | 0.182 |
| R-free | 0.24694 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b78 3ldg 3ldf |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.961 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Number of reflections | 58938 | ||
| Completeness [%] | 94.7 | 99.7 | 76.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1M HEPES, 12%(w/v) PEG 8000 , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
