3V8F
Crystal structure of crosslinked GltPh V216C-M385C mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.300, 199.810, 111.251 |
| Unit cell angles | 90.00, 117.08, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.800 |
| R-factor | 0.244 |
| Rwork | 0.243 |
| R-free | 0.25457 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.664 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 96.700 |
| High resolution limit [Å] | 3.800 |
| Number of reflections | 46875 |
| Completeness [%] | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 15 - 20% PEG 350 MME, 0.1 M Hepes, ph = 7.4, 0.15 - 0.3 M CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
