3V7O

Crystal structure of the C-terminal domain of Ebola virus VP30 (strain Reston-89)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-09
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	49.310, 93.740, 111.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.250
R-factor	0.19478
Rwork	0.193
R-free	0.22640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i8b
RMSD bond length	0.011
RMSD bond angle	1.328
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.3.0)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			2.310
High resolution limit [Å]	2.250	10.060	2.250
Rmerge	0.082	0.019	0.506
Number of reflections	25173
<I/σ(I)>	15.18	47.81	3.15
Completeness [%]	99.7	83.1	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	EbreA.17250.a at 18.3 mg/ml. 10% PEG6000, 0.1M HEPES pH 7.0, Cryoprotectant 20% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 289K