3V7M
Crystal structure of monoclonal human anti-Rhesus D Fc IgG1 T125(YB2/0) in the presence of Zn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-19 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.220, 147.750, 75.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.240 - 2.020 |
| R-factor | 0.237 |
| Rwork | 0.234 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2J6E FC PORTION ONLY. |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.136 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.140 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.063 | 0.759 |
| Number of reflections | 17681 | |
| <I/σ(I)> | 18.81 | 2.71 |
| Completeness [%] | 93.2 | 70.1 |
| Redundancy | 7.2 | 6.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.1 | 316 | 8% MPEG 5000, 300 MICRO-M ZNCL2, 100 MICRO-M NICL2, 0.1 M SODIUM CACODYLATE, pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 316KK |
