Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 54.002, 66.752, 70.287 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.000 - 1.743 |
| R-factor | 0.181 |
| Rwork | 0.177 |
| R-free | 0.22790 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.763 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.000 | 40.000 | 1.790 |
| High resolution limit [Å] | 1.750 | 4.220 | 1.750 |
| Rmerge | 0.173 | 0.108 | 0.704 |
| Number of reflections | 25734 | ||
| <I/σ(I)> | 6 | 1.84 | |
| Completeness [%] | 96.9 | 99.8 | 77.7 |
| Redundancy | 12 | 13 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 4% PEG 8000, PH 8.0, 20 mM HEPES buffer, 250 mM sodium chloride, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 291K |
