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3V7A

Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-BM
Synchrotron siteAPS
Beamline22-BM
Temperature [K]298
Detector technologyCCD
Collection date2010-08-10
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)1
Spacegroup nameP 43 2 2
Unit cell lengths145.480, 145.480, 216.334
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution31.537 - 3.297
R-factor0.2295
Rwork0.227
R-free0.28260
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3ONU for P domain i.e. chains A and B and 1WEJ for Fab i.e. chains D E F G
RMSD bond length0.004
RMSD bond angle0.795
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwarePHASES
Refinement softwarePHENIX ((phenix.refine: dev_755))
Data quality characteristics
 Overall
Low resolution limit [Å]31.537
High resolution limit [Å]3.297
Number of reflections30833
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.529840% v/v PEG 400, 5% w/v PEG 3350, and 0.1 M acetic acid pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

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