3V7A
Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2010-08-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 145.480, 145.480, 216.334 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.537 - 3.297 |
R-factor | 0.2295 |
Rwork | 0.227 |
R-free | 0.28260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ONU for P domain i.e. chains A and B and 1WEJ for Fab i.e. chains D E F G |
RMSD bond length | 0.004 |
RMSD bond angle | 0.795 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: dev_755)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 31.537 |
High resolution limit [Å] | 3.297 |
Number of reflections | 30833 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 40% v/v PEG 400, 5% w/v PEG 3350, and 0.1 M acetic acid pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |