3V7A

Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	298
Detector technology	CCD
Collection date	2010-08-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 43 2 2
Unit cell lengths	145.480, 145.480, 216.334
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.537 - 3.297
R-factor	0.2295
Rwork	0.227
R-free	0.28260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ONU for P domain i.e. chains A and B and 1WEJ for Fab i.e. chains D E F G
RMSD bond length	0.004
RMSD bond angle	0.795
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: dev_755))

Data quality characteristics

	Overall
Low resolution limit [Å]	31.537
High resolution limit [Å]	3.297
Number of reflections	30833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	40% v/v PEG 400, 5% w/v PEG 3350, and 0.1 M acetic acid pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K