3V75
Crystal structure of putative orotidine 5'-phosphate decarboxylase from Streptomyces avermitilis ma-4680
Replaces: 3L52Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-24 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.882, 83.342, 44.081 |
| Unit cell angles | 90.00, 110.52, 90.00 |
Refinement procedure
| Resolution | 27.444 - 1.400 |
| R-factor | 0.1493 |
| Rwork | 0.148 |
| R-free | 0.17210 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.520 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 53899 | |
| <I/σ(I)> | 43.24 | 3.79 |
| Completeness [%] | 93.2 | 61 |
| Redundancy | 4.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1 M TRIS PH 8.5, 0.2 M SODIUM ACETATE, 30% PEG 4K, TEV PROTEASE, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
