3V72

Crystal Structure of Rat DNA polymerase beta Mutator E295K: Enzyme-dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-14
Detector	MAR CCD 165 mm
Wavelength(s)	0.97931
Spacegroup name	P 21 21 21
Unit cell lengths	57.193, 73.763, 118.367
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.758 - 2.490
R-factor	0.223
Rwork	0.218
R-free	0.29070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.338
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.758	4.754	2.621
High resolution limit [Å]	2.490	3.777	2.490
Number of reflections	15396
Completeness [%]	94.2	99	64

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	296	PEG 3350, NaCl, Glycerol, Cacodylate, pH 6.5, vapor diffusion, temperature 296K