3V6Y

crystal structure of FBF-2 in complex with a mutant gld-1 FBEa13 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-17
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54
Spacegroup name	P 61
Unit cell lengths	99.797, 99.797, 105.598
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.207 - 2.500
R-factor	0.1877
Rwork	0.185
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.006
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.099	0.680
Number of reflections	20644
<I/σ(I)>	26.1	3.8
Completeness [%]	99.8	100
Redundancy	11.2	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	10% (w/v) PEG8000, 8% (v/v) ethylene glycol, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K