3V6T
Crystal structure of the DNA-bound dHax3, a TAL effector, at 1.85 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.719, 87.679, 88.494 |
| Unit cell angles | 90.00, 103.04, 90.00 |
Refinement procedure
| Resolution | 32.316 - 1.850 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v6p |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.157 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 103239 | |
| Completeness [%] | 99.7 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 8-10% PEG 3350(w/v), 12% ethanol, 0.1M MES pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
