3V6P

Crystal structure of the DNA-binding domain of dHax3, a TAL effector

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	C 2 2 21
Unit cell lengths	74.762, 95.507, 153.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.435 - 2.401
R-factor	0.2137
Rwork	0.211
R-free	0.26360
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.234
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	20832
Completeness [%]	95.6	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	8-12% PEG 3350(w/v), 0.1M sodium citrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	8	291	8-12% PEG 3350(w/v), 0.1M sodium citrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K