3V67
Periplasmic domain of Vibrio parahaemolyticus CpxA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-06-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97943, 0.97970, 0.98751, 0.97176 |
| Spacegroup name | H 3 |
| Unit cell lengths | 148.583, 148.583, 32.314 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.876 - 2.300 |
| R-factor | 0.2336 |
| Rwork | 0.230 |
| R-free | 0.27360 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.911 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (2011_12_05_2329) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 11802 | |
| <I/σ(I)> | 24.3 | 4.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 295 | 0.1M Tris-HCl, 18-24% PEG 3000, 0.2M Ca(OAc)2, 3%(v/v) 1,6-hexanediol, 2mM tris(2-carboxyethyl)phosphine, pH 7.0, EVAPORATION, temperature 295K |
