3V5X

Structure of FBXL5 hemerythrin domain, C2 cell

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-28
Detector	ADSC Q315
Wavelength(s)	0.98717
Spacegroup name	C 1 2 1
Unit cell lengths	60.118, 75.802, 78.727
Unit cell angles	90.00, 111.75, 90.00

Refinement procedure

Resolution	27.682 - 1.850
R-factor	0.1709
Rwork	0.169
R-free	0.20870
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.211
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.047	0.027	0.690
Number of reflections	28254
<I/σ(I)>	12.2
Completeness [%]	99.8	97.4	100
Redundancy	5	4.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	10% PEG 6000, 0.1 M HEPES, 25% ethylene glycol, pH 6.5, vapor diffusion, hanging drop, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	10% PEG 6000, 0.1 M HEPES, 25% ethylene glycol, pH 6.5, vapor diffusion, hanging drop, temperature 293K