3V5E
Crystal structure of ClpP from Staphylococcus aureus in the active, extended conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.220, 94.860, 139.020 |
| Unit cell angles | 90.00, 97.74, 90.00 |
Refinement procedure
| Resolution | 48.200 - 2.300 |
| R-factor | 0.20235 |
| Rwork | 0.201 |
| R-free | 0.23108 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qwd |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC (5.6.0119) |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.065 | 0.510 |
| Number of reflections | 129058 | |
| <I/σ(I)> | 11.21 | 2.23 |
| Completeness [%] | 96.1 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 200 mM Sodium malonate, 38% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
