3V58

Crystal Structure of the B-phycoerythrin from the red algae Porphyridium Cruentum at pH5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	H 3
Unit cell lengths	187.000, 187.000, 59.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.850
R-factor	0.1802
Rwork	0.178
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lia
RMSD bond length	0.017
RMSD bond angle	2.411
Data reduction software	MOSFLM (3.3.20)
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	93.500	19.814	1.950
High resolution limit [Å]	1.850	5.850	1.850
Rmerge	0.071	0.022	0.483
Total number of observations		9587	31314
Number of reflections	64691
<I/σ(I)>	13.1	26.3	1.6
Completeness [%]	98.0	96.5	96.7
Redundancy	3.7	4.8	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	counter-diffusion in capillary	5	298	3M ammonium sulphate, 0.1M sodium acetate, pH 5, counter-diffusion in capillary, temperature 298K