3V57

Crystal Structure of the B-phycoerythrin from the red algae Porphyridium Cruentum at pH8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	H 3
Unit cell lengths	187.110, 187.110, 59.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.1787
Rwork	0.177
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lia
RMSD bond length	0.015
RMSD bond angle	2.510
Data reduction software	MOSFLM (3.3.20)
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	93.555	19.806	1.790
High resolution limit [Å]	1.700	5.380	1.700
Rmerge	0.072	0.034	0.553
Total number of observations		11962	53036
Number of reflections	84772
<I/σ(I)>	11.6	19.4	1.4
Completeness [%]	99.6	95.1	100
Redundancy	4.3	4.7	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	counter-diffusion in capillary	8	298	3M ammonium sulphate, 0.1M Tris, pH 8, counter-diffusion in capillary, temperature 298K