3V4Q
Structure of R335W mutant of human Lamin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2010-11-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97690 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 90.499, 90.499, 75.425 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.590 - 3.060 |
| R-factor | 0.2762 |
| Rwork | 0.275 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 75.400 |
| High resolution limit [Å] | 3.060 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 15% Ethanol, 0.1M Citrate pH 5.5, 0.2 M Litium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
