3V4M

Crystal structure of a RNA binding domain of a U2 small nuclear ribonucleoprotein auxiliary factor 2 (U2AF) from Mus musculus at 1.80 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537,0.9793
Spacegroup name	P 65 2 2
Unit cell lengths	60.192, 60.192, 239.686
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.190 - 1.800
R-factor	0.1788
Rwork	0.177
R-free	0.20690
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.289
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2010)
Phasing software	SHELX
Refinement software	REFMAC (5.6.0116)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.190		1.860
High resolution limit [Å]	1.800	3.870	1.800
Rmerge	0.078	0.026	0.854
Number of reflections	25066	4570	4213
<I/σ(I)>	17.88	52.2	2.2
Completeness [%]	100.0	99.6	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	277	4.0M NaFormate, No Buffer pH 7.3, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K