3V4F
H-Ras PEG 400/CaCl2, ordered off
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.521, 88.521, 134.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.274 - 1.391 |
| R-factor | 0.1836 |
| Rwork | 0.183 |
| R-free | 0.19790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.169 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 1.410 |
| High resolution limit [Å] | 1.390 | 3.770 | 1.390 |
| Rmerge | 0.063 | 0.040 | 0.796 |
| Number of reflections | 40675 | ||
| <I/σ(I)> | 14.1 | ||
| Completeness [%] | 99.9 | 99.5 | 98.4 |
| Redundancy | 10.1 | 9.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG 3350 200 mM CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
