3V4D
Crystal structure of RutC protein a member of the YjgF family from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.255, 179.181, 45.217 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.1946 |
| Rwork | 0.193 |
| R-free | 0.21658 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.707 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.094 | 0.633 |
| Number of reflections | 55593 | |
| <I/σ(I)> | 34.3 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.6 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.1 M MMT, 30% PEG 1500, +xylitol, K/Na tartrate, 2,6-pyridinecarboxylic acid, 2,4-pyridinecarboxylic acid, 2,5-pyridinecarboxylic acid, 4-pyridazine carboxylic acid, 3-sulfobenzoic acid, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
