3V4B
Crystal structure of an enolase from the soil bacterium Cellvibrio japonicus (TARGET EFI-502161) with bound MG and L-tartrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-15 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9793 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 124.715, 124.715, 110.871 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.136 - 1.400 |
R-factor | 0.1493 |
Rwork | 0.149 |
R-free | 0.16180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qjm |
RMSD bond length | 0.017 |
RMSD bond angle | 1.628 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 88.187 | 88.187 | 1.480 |
High resolution limit [Å] | 1.400 | 4.430 | 1.400 |
Rmerge | 0.071 | 0.023 | 0.623 |
Total number of observations | 40004 | 156024 | |
Number of reflections | 85453 | ||
<I/σ(I)> | 25.2 | 26.1 | 1.3 |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 13.9 | 13.7 | 12.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop vapor diffusion | 4.6 | 298 | Protein (10 mM Hepes, pH 7.8, 150 mM NaCl, 10% glycerol, 5 mM DTT, 5 mM MgCl2; Reservoir (0.2M KNaTartrate, 20% Peg3350); Cryoprotection (20% glycerol), sitting drop vapor diffusion, temperature 298K |