3V43
Crystal structure of MOZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-30 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.080, 57.630, 76.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.130 - 1.470 |
| R-factor | 0.1541 |
| Rwork | 0.153 |
| R-free | 0.17620 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.386 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.255 | 38.255 | 1.550 |
| High resolution limit [Å] | 1.467 | 4.640 | 1.470 |
| Rmerge | 0.035 | 0.456 | |
| Rmeas | 0.051 | 0.493 | |
| Rpim | 0.014 | 0.135 | |
| Total number of observations | 11803 | 49207 | |
| Number of reflections | 27192 | ||
| <I/σ(I)> | 24 | 47.8 | 5.8 |
| Completeness [%] | 99.6 | 98.1 | 97.8 |
| Redundancy | 13.6 | 12.3 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2M ammonium acetate, 0.1M tri-sodium citrate, 30% w/v PEG 4000, pH 5.6, vapor diffusion, hanging drop, temperature 293K |
