3V42
Crystal structure of renal tumor suppressor protein, folliculin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-25 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 86.050, 99.951, 107.584 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.140 - 2.000 |
R-factor | 0.207 |
Rwork | 0.204 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SEMET STRUCTURE OF FOLLICULIN COLLECTED AT 2.9A |
RMSD bond length | 0.024 |
RMSD bond angle | 1.925 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.850 | 1.970 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.073 | 0.648 |
Number of reflections | 35477 | |
<I/σ(I)> | 14.3 | 2.1 |
Completeness [%] | 97.6 | 61.2 |
Redundancy | 6.1 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100 MM BIS-TRIS, PH5.5, 200 MM LISO4, 25% PEG 3350, VAPOR DIFFUSION, TEMPERATURE 293K, VAPOR DIFFUSION, HANGING DROP |