3V3T
Crystal structure of Clostridium botulinum phage c-st TubZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9393 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.990, 85.606, 44.935 |
| Unit cell angles | 90.00, 93.87, 90.00 |
Refinement procedure
| Resolution | 44.833 - 2.302 |
| R-factor | 0.1698 |
| Rwork | 0.167 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.286 | 44.833 | 2.420 |
| High resolution limit [Å] | 2.302 | 7.270 | 2.302 |
| Rmerge | 0.033 | 0.417 | |
| Total number of observations | 4198 | 9951 | |
| Number of reflections | 16843 | ||
| <I/σ(I)> | 22.9 | 15.7 | 1.9 |
| Completeness [%] | 95.5 | 99.4 | 73.4 |
| Redundancy | 7.2 | 7.2 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 17% PEG 10000, 0.1M ammonium acetate, 0.1M BisTris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
