3V3L
Crystal structure of human RNF146 WWE domain in complex with iso-ADPRibose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-21 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 55.343, 55.343, 146.471 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.650 |
R-factor | 0.153 |
Rwork | 0.151 |
R-free | 0.18100 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.573 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.680 |
High resolution limit [Å] | 1.630 | 1.630 |
Number of reflections | 32399 | |
Completeness [%] | 96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 295 | 45% PEG 400, 100 mM Tris HCl pH 8.0, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 295K |