3V39
Bd3459, A Predatory Peptidoglycan Endopeptidase from Bdellovibrio bacteriovorus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 125.240, 125.240, 81.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.154 - 1.450 |
| R-factor | 0.1669 |
| Rwork | 0.166 |
| R-free | 0.18180 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.918 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.460 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 554908 | |
| Completeness [%] | 98.6 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1M Mes, 0.2M Ammonium Sulphate, 30% w/v PEG 5000MME 16% v/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 6.5 |
