3V30
Crystal Structure of the Peptide Bound Complex of the Ankyrin Repeat Domains of Human RFXANK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97924 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.804, 31.499, 63.079 |
Unit cell angles | 90.00, 107.53, 90.00 |
Refinement procedure
Resolution | 46.880 - 1.570 |
R-factor | 0.1656 |
Rwork | 0.164 |
R-free | 0.19540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3so8 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.208 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (refmac_5.6.0081) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.610 |
High resolution limit [Å] | 1.570 | 3.870 | 1.570 |
Rmerge | 0.039 | 0.018 | 0.236 |
Number of reflections | 28759 | ||
<I/σ(I)> | 27.2 | ||
Completeness [%] | 99.7 | 99.7 | 97.6 |
Redundancy | 4.5 | 4.4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1M Bis-Tris pH 6.5, 0.2 M ammonium acetate, 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |