3V2X
Crystal Structure of the Peptide Bound Complex of the Ankyrin Repeat Domains of Human ANKRA2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-07 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 |
Unit cell lengths | 29.962, 32.750, 45.314 |
Unit cell angles | 78.51, 75.17, 65.95 |
Refinement procedure
Resolution | 43.550 - 1.850 |
R-factor | 0.1823 |
Rwork | 0.181 |
R-free | 0.21270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3so8 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.171 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (refmac_5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.890 |
High resolution limit [Å] | 1.850 | 4.560 | 1.850 |
Rmerge | 0.043 | 0.033 | 0.171 |
Number of reflections | 11884 | ||
<I/σ(I)> | 18 | ||
Completeness [%] | 92.1 | 98.8 | 69.1 |
Redundancy | 3.8 | 3.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1M Bis-Tris, pH 6.5, 0.2M NaCl, 25% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |