3V2U

Crystal structure of the yeast GAL regulon complex of the repressor, Gal80p, and the transducer, Gal3p, with galactose and ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-16
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 1
Unit cell lengths	82.186, 83.221, 92.629
Unit cell angles	114.04, 92.88, 90.37

Refinement procedure

Resolution	47.634 - 2.105
R-factor	0.1505
Rwork	0.150
R-free	0.19650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Gal80p (1-435) 3BTS Gal3p (259-520) 3V5R
RMSD bond length	0.007
RMSD bond angle	1.001
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge		0.041	0.480
Number of reflections	126106
<I/σ(I)>		4.695	1.585
Completeness [%]	97.5	99.1	96.5
Redundancy		4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	290	10% PEG 3350, 0.1M di-sodium succinate, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K