3V2O
Crystal Structure of the Peptide Bound Complex of the Ankyrin Repeat Domains of Human ANKRA2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.266, 49.569, 41.059 |
| Unit cell angles | 90.00, 97.52, 90.00 |
Refinement procedure
| Resolution | 40.710 - 1.890 |
| R-factor | 0.2287 |
| Rwork | 0.227 |
| R-free | 0.26930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3so8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.146 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.890 | 4.090 | 1.890 |
| Rmerge | 0.096 | 0.071 | 0.263 |
| Number of reflections | 11646 | ||
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 95.1 | 98.7 | 76.8 |
| Redundancy | 4.3 | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M Hepes, pH 7.5, 0.2M ammonium acetate, 25% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
