3V2L
Structure of Anopheles gambiae odorant binding protein 20 bound to polyethylene glycol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-29 |
Detector | NOIR-1 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.577, 38.229, 89.912 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.000 - 1.800 |
R-factor | 0.1991 |
Rwork | 0.194 |
R-free | 0.24230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.218 |
Data reduction software | d*TREK (9.9.7LDz) |
Data scaling software | d*TREK (9.9.7LDz) |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.230 | 38.230 | 1.660 |
High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
Rmerge | 0.082 | 0.077 | 0.429 |
Total number of observations | 6772 | 6679 | |
Number of reflections | 11263 | ||
<I/σ(I)> | 7.7 | 18.2 | 2.5 |
Completeness [%] | 98.8 | 94.4 | 100 |
Redundancy | 4.13 | 4 | 4.17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 276 | 29% PEG 3350, 0.1M sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 276K |