3V2I
Structure of a Peptidyl-tRNA hydrolase (PTH) from Burkholderia thailandensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-17 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 53.340, 93.580, 106.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.650 |
R-factor | 0.1767 |
Rwork | 0.175 |
R-free | 0.20550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ofv |
RMSD bond length | 0.010 |
RMSD bond angle | 1.605 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.690 | |
High resolution limit [Å] | 1.650 | 7.380 | 1.650 |
Rmerge | 0.041 | 0.014 | 0.422 |
Number of reflections | 32529 | 390 | 2386 |
<I/σ(I)> | 30.89 | 72.97 | 4.89 |
Completeness [%] | 99.9 | 91.3 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | ButhA.17877.a.A1 PS01195 34.03 mg/ml, 1000mM sodium citrate, 100mM cacodylate pH 6.5. 25% ethylene glycol for cryoprotection. , VAPOR DIFFUSION, SITTING DROP, temperature 289K |