3V1Y
Crystal structures of glyceraldehyde-3-phosphate dehydrogenase complexes with NAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.171, 128.173, 77.314 |
Unit cell angles | 90.00, 117.38, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.860 |
R-factor | 0.267 |
Rwork | 0.234 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.860 |
Number of reflections | 108982 |
Completeness [%] | 99.2 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 291 | 16% PEG 3350 (w/v), 0.1M citric acid pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K |