3V11
Structure of the ternary initiation complex AIF2:GDPNP:methionylated initiator TRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.12713 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 133.000, 133.000, 167.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.426 - 5.000 |
| R-factor | 0.2654 |
| Rwork | 0.262 |
| R-free | 0.34380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2AHO and 3CW5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.557 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 115.000 | 5.200 |
| High resolution limit [Å] | 5.000 | 5.000 |
| Rmerge | 0.066 | 0.847 |
| Number of reflections | 7664 | |
| Completeness [%] | 98.9 | 99.9 |
| Redundancy | 6.4 | 6.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 277 | 5% PEG 20 000, 50mM MES 5.7, 200mM KCl, 10mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
