3V10
Crystal structure of the collagen binding domain of Erysipelothrix rhusiopathiae surface protein RspB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.170, 67.390, 102.410 |
| Unit cell angles | 90.00, 92.82, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| Rwork | 0.225 |
| R-free | 0.26100 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw (software pipeline) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.000 |
| High resolution limit [Å] | 1.750 |
| Rmerge | 0.055 |
| Number of reflections | 112956 |
| Completeness [%] | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 35% PEG 2000 monomethyl ether, 100mM sodium cacodylate, 20mM MgCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
