3V0V
Fab WN1 222-5 unliganded
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-29 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.886, 48.201, 140.131 |
| Unit cell angles | 90.00, 110.55, 90.00 |
Refinement procedure
| Resolution | 19.985 - 2.130 |
| R-factor | 0.2402 |
| Rwork | 0.238 |
| R-free | 0.28430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yef |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.733 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.985 | 19.890 | 2.800 |
| High resolution limit [Å] | 2.130 | 5.770 | 2.700 |
| Rmerge | 0.062 | 0.044 | 0.220 |
| Total number of observations | 8707 | 6271 | |
| <I/σ(I)> | 13.1 | 23.9 | 3.9 |
| Completeness [%] | 93.6 | 99.9 | 85 |
| Redundancy | 3.18 | 3.39 | 3.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | HEPES, PEG 3350, Propanol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
