3V0F
Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 241-576(C363S), form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1158 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.746, 80.375, 83.637 |
Unit cell angles | 90.00, 93.12, 90.00 |
Refinement procedure
Resolution | 83.510 - 1.300 |
R-factor | 0.14156 |
Rwork | 0.140 |
R-free | 0.17502 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB: 3V0D |
RMSD bond length | 0.010 |
RMSD bond angle | 1.354 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.000 | 1.330 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.084 | 0.584 |
Number of reflections | 153889 | |
<I/σ(I)> | 26.3 | 3.5 |
Completeness [%] | 94.1 | 90.1 |
Redundancy | 12.5 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 17.5-22.5% PEG 2000, 0.1 M ammonium dihydrophosphate, 0.1 M Tris-HCl,pH 8.0; crystal were soaked in 30% PEG 2000, 0.1 M ammonium dihydrophosphate, 0.1 M Tris-HCl, pH 8.0 to remove phosphate by serial dilution, VAPOR DIFFUSION, HANGING DROP, temperature 277K |