3UZU
The structure of the Ribosomal RNA small subunit methyltransferase A from Burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.190, 49.340, 61.160 |
| Unit cell angles | 90.00, 95.91, 90.00 |
Refinement procedure
| Resolution | 43.343 - 1.750 |
| R-factor | 0.1823 |
| Rwork | 0.180 |
| R-free | 0.21720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qyr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.489 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.343 | 1.800 | |
| High resolution limit [Å] | 1.750 | 7.830 | 1.750 |
| Rmerge | 0.085 | 0.022 | 0.434 |
| Number of reflections | 27262 | 336 | 2000 |
| <I/σ(I)> | 13.7 | 36.74 | 3.13 |
| Completeness [%] | 99.3 | 99.1 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 20% PEG3350, 150mM DL Malic Acid. Cryoprotectant 20% ethylene glycol. 47.14 mg/ml BupsA.01122.a.A1 PS01140, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
